Study on the clinical effect of minimally invasive mastectomy with Mammotome in the treatment of breast benign tumor / 中国基层医药

Objective:To study the clinical effect of Mammotome minimally invasive breast circumcision in the treatment of breast cancer.Methods:A prospective study was carried out in 800 breast benign tumor patients admitted to the People's Hospital of Anji County from January 2017 to December 2018.The patients were divided into control group(400 cases) and observation group(400 cases) according to the random digital table method.The control group received open breast tumor resection, and the observation group received minimally invasive mastectomy.The postoperative complications, recovery time, aesthetic effect of breast appearance, serum inflammatory factors and cellular immune function were compared between the two groups.Results:The operation time and bleeding volume in the observation group were (17.59±8.23)min, (23.57±7.64)mL, respectively, which were lower than those in the control group [(29.83±11.91)min, (54.28±17.59)mL]( t=16.910, 30.027, all P<0.05). The incidence of postoperative complications in the observation group was 1.50%(6/400), which was lower than 5.75%(23/400) in the control group(χ 2=10.340, P<0.05). The time of incision healing and hospitalization in the observation group were (4.07±1.39)d, (5.58±2.31)d, respectively, which were shorter than those in the control group [(5.56±1.75)d, (7.92±2.78)d]( t=13.334, 12.948, all P<0.05). The excellent and good rate of breast appearance aesthetics in the observation group was 97.25%(389/400), which was higher than 89.25%(357/400) in the control group(χ 2=20.336, P<0.05). After operation, the serum levels of inflammatory factors in the observation group were lower than those in the control group( t=17.360, 14.717, 14.109, all P<0.05). CD 3+ and CD 4+ /CD 8+ in the two groups were lower than before operation(control group: t=20.788, 16.121; observation group: t=11.616, 8.348, all P<0.05), and CD 3+ and CD 4+ /CD 8+ in the observation group were higher than those in the control group( t=10.655, 7.328, all P<0.05). Conclusion:Mammotome minimally invasive mastectomy can effectively reduce the surgical trauma of breast cancer patients, reduce the postoperative complications, reduce the impact on their cellular immune function, and help to speed up the recovery and improve the appearance of breast.

Anticoagulant Ability and Heparinization of Decellularized Biomaterial Scaffolds / 生物医学工程学杂志

In order to enhance the anticoagulant properties of decellularized biological materials as scaffolds for tissue engineering research via heparinized process, the decellularized porcine liver scaffolds were respectively immobilized with heparin through layer-by-layer self-assembly technique (LBL), multi-point attachment (MPA) or end-point attachment (EPA). The effects of heparinization and anticoagulant ability were tested. The results showed that the three different scaffolds had different contents of heparin. All the three kinds of heparinized scaffolds gained better performance of anticoagulant than that of the control scaffold. The thrombin time (TT), prothrombin time (PT) and activated partial thromboplastin time (APTT) of EPA scaffold group were longest in all the groups, and all the three times exceeded the measurement limit of the instrument. In addition, EPA scaffolds group showed the shortest prepared time, the slowest speed for heparin release and the longest recalcification time among all the groups. The decellularized biological materials for tissue engineering acquire the best effect of anticoagulant ability in vitro via EPA heparinized technique.

Establishment of TaqManˉBHQ probe realˉtime fluorescence RTˉPCR for quantitative detection of Soul virus / 国际检验医学杂志

Objective To establish a rapid method of real-time fluorescence RT-PCR to quantify Soul virus.Methods The pro-fessional software was adopted to design the primer and the TaqMan-BHQ probe.With artificially synthesized L gene segment as the template of Soul virus,the real-time RT-PCR for detecting Soul virus was researched.Results The Ct value of templates had a good linear relationship with the log value of the template diluted concentration.The standard curve was Y =-3.607X +41.84, r2 =0.998,the PCR amplification efficiency was 108.1%,its lowest detection limit was 53.2 copies/μL.Conclusion Applying the real-time fluorescence RT-PCR by the TaqMan-BHQ probe for detecting nucleic acid of Seoul virus has the characteristics of short time-consuming and high sensitivity.

Ectopic Over-expression of Oncogene Pim-2 Induce Malignant Transformation of Nontumorous Human Liver Cell Line L02

In order to prove that ectopic over-expression of Pim-2 could induce malignant transformation of human liver cell line L02, three groups of cells were set up including human liver cell line L02 (L02), L02 cells transfected with Pim-2 gene (L02/Pim-2) and L02 cells transfected with empty-vector (L02/Vector). Pim-2 expression levels were detected. The morphology, proliferation level, apoptosis rate and migration ability of the cells were detected respectively. Then the cells were subcutaneously inoculated into athymic mice and the microstructures of the neoplasm were observed. Compared with the controls, Pim-2 expression levels were significantly higher in L02/Pim-2 cells (P<0.05), and their morphology had obvious malignant changes. They also showed a significantly increased proliferation rate (P<0.05) and migration capacity (P<0.05), as well as a significantly decreased apoptosis rate (P<0.05). Only the athymic mice inoculated with L02/Pim-2 cells could generate neoplasm, and the morphology of the neoplasm coincided with that of the hepatoma. The results manifest that ectopic Pim-2 gene could be stably expressed in L02/Pim-2 cells. Both the morphological and biological changes of L02/Pim-2 cells demonstrate the trend of malignant transformation. L02/Pim-2 cells could generate hepatoma in athymic mice. In conclusion, Pim-2 could induce malignant transformation of human liver cell line L02.

Construction of recombinant adenovirus vector expressing EGFP gene specifically in AFP producing liver cancer cells / 中国病理生理杂志

AIM: To construct a recombinant adenovirus vector carrying AFP promoter to specifically express a targeting gene in hepatocellular carcinoma HepG2 cells. METHODS: Based on the Adeno-X~TM Expression system, the CMV promoter was replaced by a 300 bp a-fetoprotein promoter. The EGFP(enhanced green fluorescent protein) gene as a report gene was inserted to the multiple-cloning site(MCS). The normal liver LO2 cells, hepatocellular carcinoma HepG2 cells and HeLa cells were infected by the recombinant adenovirus, respectively. Northern blotting and fluorescence microscope were used to detect the transcription level of EGFP gene and its protein expression, respectively. RESULTS: Northern blotting showed that the target gene was markedly transcribed in HepG2 cells, but slightly in LO2 and HeLa cells. Under the fluorescence microscope, strong EGFP expression was seen in HepG2 cells but very weakly in HeLa and LO2 cells. CONCLUSION: Under the control of the 300 bp human AFP promoter, the target gene carried by the recombinant adenovirus was expressed in the AFP-producing HepG2 cells at a very high level, but not or very weakly in AFP negative cells. This adenovirus system can be used as a new, potent and specific approach for the gene-targeting therapy for the AFP producing primary hepatoma. [